Journal of the Chinese Medical Association
Volume 70, Issue 4 , Pages 152-158, April 2007

Alpha 2,6-Sialyltransferase I Expression in the Placenta of Patients with Preeclampsia

  • Peng-Hui Wang

      Affiliations

    • Department of Obstetrics and Gynecology, Taipei Veterans General Hospital, Taipei, Taiwan, R.O.C.
    • Institute of Clinical Medicine, National Yang-Ming University School of Medicine, Taipei, Taiwan, R.O.C.
    • Corresponding Author InformationCorrespondence to: Dr Peng-Hui Wang, Department of Obstetrics and Gynecology, Taipei Veterans General Hospital, 201, Section 2, Shih-Pai Road, Taipei 112, Taiwan, R.O.C.
    • ,
  • Wen-Ling Lee

      Affiliations

    • Institute of Clinical Medicine, National Yang-Ming University School of Medicine, Taipei, Taiwan, R.O.C.
    • Department of Medicine, Cheng Hsin Rehabilitation Center, Taipei, Taiwan, R.O.C.
    • ,
  • Yu-Hui Yang

      Affiliations

    • Department of Obstetrics and Gynecology, Taipei Veterans General Hospital, Taipei, Taiwan, R.O.C.
    • Institute of Clinical Medicine, National Yang-Ming University School of Medicine, Taipei, Taiwan, R.O.C.
    • ,
  • Yi-Jen Chen

      Affiliations

    • Department of Obstetrics and Gynecology, Taipei Veterans General Hospital, Taipei, Taiwan, R.O.C.
    • Institute of Clinical Medicine, National Yang-Ming University School of Medicine, Taipei, Taiwan, R.O.C.
    • ,
  • Ying-Chieh Tsai

      Affiliations

    • Institute of Biochemistry, National Yang-Ming University, Taipei, Taiwan, R.O.C.
    • ,
  • Chiou-Chung Yuan

      Affiliations

    • Department of Obstetrics and Gynecology, Taipei Veterans General Hospital, Taipei, Taiwan, R.O.C.

Received 8 June 2006; accepted 9 March 2007.

Article Outline

Background

The expression of sialyl-glycoconjugates changes during development, differentiation and oncogenic transformation, tumor invasion and metastasis. Similarly, in the early stage of pregnancy, trophoblast cells have to undergo adhesion, invasion, and proliferation to develop a healthy placenta; the cytobiologic behavior is similar to tumor growth and invasion. Inadequate trophoblast invasion to the spiral artery in the 2nd trimester of pregnancy was believed to be correlated with pregnancy complications, including preeclampsia.

Methods

Alterations in α2,6-sialyltransferase I (ST6Gal I) mRNA in the placental tissues of women with preeclampsia (n = 20) and without preeclampsia (n = 20 used as a control) were examined by semiquantitative reverse transcription-polymerase chain reaction and real-time quantitative reverse transcription-polymerase chain reaction. The transcription regulators of ST6Gal I including a “constitutive” promoter (Y + Z form), “hepatic” promoter (H form), and lymphoblastic promoter (X form) were investigated. The enzyme activity of ST6Gal I was also examined.

Results

Both mRNA expression and enzyme activity of ST6Gal I did not show a significant difference in the placental tissues of the women of both groups. The transcription regulators of ST6Gal I, including the Y + Z form and the H form, also failed to show any difference. The X form, seldom detected in the study, was excluded from analysis.

Conclusion

Our results suggested that ST6Gal I was not involved in the pathogenesis of the preeclampsia.

Key Words:  α2 , 6-sialyltransferase I , placenta , preeclampsia , pregnancy

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PII: S1726-4901(09)70349-1

doi:10.1016/S1726-4901(09)70349-1

Journal of the Chinese Medical Association
Volume 70, Issue 4 , Pages 152-158, April 2007

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