Journal of the Chinese Medical Association
Volume 69, Issue 5 , Pages 207-212, May 2006

Effects of Cinnamic Acid on Expression of Tissue Factor Induced by TNFα in Endothelial Cells and its Mechanisms

  • Xiaofei Li
    • ,
  • Zhibin Wen

      Affiliations

    • Corresponding Author InformationCorrespondence to: Dr. Zhibin Wen, Hemostasis Physiology Laboratory, Department of Physiology, Xiangya School of Medicine, Central South University, Changsha, Hunan 410078, P.R. China
    • ,
  • Xiaofan He
    • ,
  • Shilin He

Hemostasis Physiology Laboratory, Department of Physiology, Xiangya School of Medicine, Central South University, Changsha, Hunan, P.R. China

Received 21 June 2005; accepted 20 March 2006.

Article Outline

Background

To observe the effects of cinnamic acid (CINN) on the expression of tissue factor (TF) induced by tumor necrosis factor alpha (TNFα) in human umbilical vein endothelium-derived cell line (ECV304) and the related mechanisms.

Methods

Cellular TF activity was determined with 1-stage clotting assay in the presence or absence of anti-TF antibody or DEGR-VIIa. TF mRNA was examined by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR). I-κB was measured by Western blot analysis. Immunohistochemical analysis was performed to evaluate the activation of NF-κB.

Results

CINN (500 μg/mL) significantly decreased the TF activity in ECV304 induced by TNFα (1,000 U/mL) (p< 0.01) in a concentration-dependent fashion (r = −0.953, p < 0.05). CINN also inhibited the expression of TF mRNA induced by TNFα. Immunohistochemical analysis demonstrated NF-κB translocation from the cytoplasm to the nucleus, and Western blot analysis showed I-?B decrement in cytoplasm after treatment of endothelial cells with TNFα. CINN diminished these changes induced by TNFα.

Conclusion

CINN is able to attenuate TNFα-induced TF expression of vascular endothelial cells by inhibiting NF-κB activation.

Key Words:  cinnamic acid , NF-κB , tissue factor , tumor necrosis factor alpha , vascular endothelial cell

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References 

  1. Tousoulis D , Davies G , Stefanadis C , Toutouzas P , Ambrose JA . Inflammatory and thrombotic mechanisms in coronary atherosclerosis . Heart . 2003;89:993–997
  2. He M, Wen Z, He X, Xiong S, Liu F, Xu J, et al  Observation on tissue factor pathway and some other coagulation parameters during the onset of acute cerebrocardiac thrombotic diseases . Thromb Res. . 2002;107:223–228
  3. Chen F , Fu Y , Zou L , Lu X , Wang C . Protection of CINN on ischemia/reperfusion injury . Zhongguo Yi Yao Xue Bao . 1999;14:68–69
  4. Cartron E , Carbonneau MA , Fouret G , Descomps B , Leger CL . Specific antioxidant activity of caffeoyl derivatives and other natural phenolic compounds: LDL protection against oxidation and decrease in the proinflammatory lysophosphatidylcholine production . J Nat Prod . 2001;64:480–486
  5. Banskota AH, Nagaoka T, Sumioka LY, Tezuka Y, Awale S, Midorikawa K, et al  Antiproliferative activity of the Netherlands propolis and its active principles in cancer cell lines . J Ethnopharmacol . 2002;80:67–73
  6. Hindman BJ . Emboli, inflammation, and CNS impairment: an overview . Heart Surg Forum . 2002;5:249–253
  7. Hathcock J . Vascular biology: the role of tissue factor . Semin Hematol . 2004;41(Suppl):30–34
  8. Iochmann S, Reverdiau-Moalic P, Beaujean S, Rideau E, Lebranchu Y, Bardos P, et al. Fast detection of tissue factor and tissue factor pathway inhibitor messenger RNA in endothelial cells and monocytes by sensitive reverse transcription-polymerase chain reaction . Thromb Res. . 1999;94:165–173
  9. Cirino G , Napolic C , Bucci M , Cicala C . Inflammation-coagulation network: are serine protease receptors the knot? . Trends Pharmacol Sci. . 2000;21:170–172
  10. Levi M , van der Poll T , Buller HR . Bidirectional relation between inflammation and coagulation . Circulation . 2004;109:2698–2704
  11. Atamas SP . Complex cytokine regulation of tissue fibrosis . Life Sci. . 2002;72:631–643
  12. Shebuski RJ , Kilgore KS . Role of inflammatory mediators in thrombogenesis . J Pharmacol Exp Ther . 2002;300:729–735
  13. Baldwin AS . The NF-kappa B and I kappa B proteins: new discoveries and insights . Annu Rev Immunol . 1996;14:649–683
  14. Lee JI , Burckart GJ . Nuclear factor kappa B: important transcription factor and therapeutic target . J Clin Pharmacol . 1998;38:981–993

PII: S1726-4901(09)70220-5

doi:10.1016/S1726-4901(09)70220-5

Journal of the Chinese Medical Association
Volume 69, Issue 5 , Pages 207-212, May 2006

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