Journal of the Chinese Medical Association
Volume 71, Issue 2 , Pages 66-73, February 2008

Comparison of the Effectiveness of Autologous Fibrin Glue and Macroporous Biphasic Calcium Phosphate as Carriers in the Osteogenesis Process With or Without Mesenchymal Stem Cells

  • Li-Tzu Lee

      Affiliations

    • Departments of Oral and Maxillofacial Surgery, Taichung, Taipei, Taiwan, R.O.C.
    • ,
  • Po-Cheung Kwan

      Affiliations

    • Departments of Pathology, Taichung Veterans General Hospital, Taichung, Taipei, Taiwan, R.O.C.
    • ,
  • Ya-Fang Chen

      Affiliations

    • Departments of Oral and Maxillofacial Surgery, Taichung, Taipei, Taiwan, R.O.C.
    • ,
  • Yong-Kie Wong

      Affiliations

    • Departments of Oral and Maxillofacial Surgery, Taichung, Taipei, Taiwan, R.O.C.
    • School of Dentistry, National Yang-Ming University, Taipei, Taiwan, R.O.C.
    • Corresponding Author InformationCorrespondence to: Dr Yong-Kie Wong, Department of Oral and Maxillofacial Surgery, Taichung Veterans General Hospital, 160, Section 3, Taichung-Kang Road, Taichung 407, Taiwan, R.O.C

Received 24 September 2007; accepted 23 January 2008.

Background

Facial bone reconstruction has been a challenge for oral and maxillofacial surgeons for a long time. Recently, some studies have reported the use of stem cells in facial reconstruction to achieve osteogenesis. However, to ensure that stem cells remain in the recipient site, a biocompatible carrier is needed to transfer the stem cells. Fibrin glue has been shown to promote hemostasis in wound management and accelerate soft tissue healing, but the role of fibrin glue in bone regeneration remains debatable. The purpose of this study was to compare the effectiveness of autologous fibrin glue and macroporous biphasic calcium phosphate (MBCP) as carriers in the osteogenesis process with/without mesenchymal stem cells.

Methods

Fifteen New Zealand white rabbits were used in this study. Mesenchymal stem cells were harvested from the iliac bone, and autologous fibrin glue was made from peripheral blood. Three cranial defects with a diameter of 6 mm were created over the cranial bone in each rabbit. The 15 animals were separated into 2 groups. The first group contained 12 rabbits. The grafted substances placed over the regions of defect were: (1) stem cells plus autologous fibrin glue; (2) stem cells plus MBCP; (3) defect alone as control. In the second group of 3 rabbits, the cranial defects were grafted with: (1) autologous fibrin glue alone; (2) MBCP alone; (3) defect alone as control. Rabbits were sacrificed at 1, 2 and 3 months post operation. Radiography and histology were used to detect bone formation.

Results

Stem cells plus autologous fibrin glue induced more bone formation 2 months post operation and more mature bone was found 3 months post operation compared with the other groups. MBCP with or without stem cells showed moderate tissue reaction, including giant cell, histiocyte and eosinophil cell accumulation.

Conclusion

Using stem cells plus autologous fibrin glue as the carrier may accelerate new bone regeneration.

Key Words:  autologous fibrin glue , biphasic calcium phosphate , osteogenesis , stem cells

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PII: S1726-4901(08)70077-7

doi:10.1016/S1726-4901(08)70077-7

Journal of the Chinese Medical Association
Volume 71, Issue 2 , Pages 66-73, February 2008

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